[Aerobic bacterial microbiota of the conjunctiva of cornea donors].
نویسندگان
چکیده
PURPOSE To determine aerobic bacterial microbiota of the conjunctiva of cornea donors and its patterns of susceptibility to antibiotics; verify the number of corneas used for transplant and the average time of preservation in solutions with gentamicin and streptomycin; trace the profile of donors and receptors of cornea. METHODS Clinical specimens were collected from the inferior bag of the conjunctiva of both eyes of 40 cornea donors. The samples were inoculated into acid blood, chocolate and MacConkey agars, and the antibiogram was performed by the Kirby-Bauer method. RESULTS The frequency of positive cultures of the conjunctiva of cornea donors was 72.5%, with Gram-positive totalling 81.6% and only 18.4% of the samples were identified as Gram-negative. Vancomycin inhibited 100% of Gram-positive microorganisms, while sensitivity of the Gram-negative to gentamicin was 53.8% and to streptomycin 30%. Most donors and recipients were men, the average time between death and enucleation was approximately 2 hours and preservation in solution with gentamicin and streptomycin was around 7 days. Neoplasms and more than one associated cause were the most frequently causes of death. Keratoconus was the main indication for transplant (51.7%). CONCLUSIONS Coagulase-negative Staphylococcus was the most frequently isolated microorganism, presenting variable sensitivity to antimicrobians. The amount of corneas used for transplant was below the number of available corneas. Donor and receptor profiles were very heterogeneous regarding most of the analyzed variables.
منابع مشابه
Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19
Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...
متن کاملAerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil.
PURPOSE To study the aerobic bacterial microbiota of the conjunctiva in diabetic patients with regard to the management of diabetes, assessed using glycated hemoglobin levels. METHODS A cross-sectional study was conducted using conjunctival smears of diabetic patients from both sexes and with different ages, residing in two different Brazilian cities (Sorocaba and Rio Branco). A control group...
متن کاملBacterial Flora Changes in Conjunctiva of Rats with Streptozotocin-Induced Type I Diabetes
BACKGROUND The microbiota of both humans and animals plays an important role in their health and the development of disease. Therefore, the bacterial flora of the conjunctiva may also be associated with some diseases. However, there are no reports on the alteration of bacterial flora in conjunctiva of diabetic rats in the literature. Therefore, we investigated the changes in bacterial flora in ...
متن کاملKeratin 13 is a more specific marker of conjunctival epithelium than keratin 19
PURPOSE To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. METHODS Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea were iden...
متن کاملThe effect of Ophthalmic Patch on Conjunctiva of Neonates under Phototherapy
Objective Conjunctivitis is a common disease in neonates. Several bacterial and viral infectious agents may cause it. Tear circulation and blinking has protective effects from bacterial colonization in the conjuntival sac. Reduced blinking increases the possibility of bacterial conjunctivitis. We studied the effect of eye patches on bacterial colonization of the conjunctival sac. Methods In 1...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Arquivos brasileiros de oftalmologia
دوره 70 2 شماره
صفحات -
تاریخ انتشار 2007